پنجشنبه 2 آذر 1396
به سایت مجله پزشکی دانشگاه علوم پزشکی و خدمات بهداشتی و درمانی تبریز خوش آمدید
   نوامبر 23, 2017
   
   
     
  تعداد اعضاء : 2866 نفر
  بازدیدهای امروز :188 بار
  تعداد کل بازدیدها : 968102 بار
   
Volume: 38
Year: 2016
Month: 9
Subject Others
Title Cassette Chromosome mec Typing of Methicillin-Resistant Staphylococcus aureus Isolates Collected from Sina and Imam Reza Hospitals of Tabriz
Authors Peyman Bohlouli; Mohammad Reza Nahaei; Safar Farajnia; Mojtaba Varshochi; Morteza Ghojazadeh; Mohammad Akbari Dibavar; Javid Sadeghi;
Abstract Backgrounds and Objectives: Methicillin-resistant Staphylococcus aureus is a cause of nosocomial infections leading to high mortality. Since these strains have become prevalent in the world, it is necessary to identify and type them. Materials and Methods: This cross-sectional study was conducted to study a total of 1475 specimens collected from patients of Imam Reza and Sina hospitals of Tabriz in 2012-2013. Using phenotypic tests such as Gram stain, catalase, coagulase, DNase and mannitol fermentation 169 isolates of Staphylococcus aureus and by utilizing methicillin-resistance test 100 MRSA isolates were identified. SCCmec typing was performed by multiplex PCR method and the results were analyzed using chi-square tests using SPSS-18 software. Results: Disc agar diffusion test using cefoxitin disc (30 µg) showed methicillin resistance in 59% of our isolates. mecA and femB genes were identified in all of the MRSA isolates using PCR method. Frequency of SCCmec types and sub-types were as follow: SCCmecIII (77%), SCCmecI (5%), SCCmecIVa (1%), SCCmecIVc (1%), mixed isolates SCCmecIVc-III (1%) and Non typeable isolates (15%). Non typeable isolates recovered in two groups (10% without any band and 5% of multi-bands III-I). In this study, 82% of isolates were HA-MRSA, 3% were CA-MRSA and 15% were Non-typeable. Conclusion: In our S. aureus isolates, the prevalence of methicillin resistance was 59%. The most frequent SCCmec type was SCCmecIII (77%). Our results demonstrated the spread of HA-MRSA isolates in the community and propagating CA-MRSA isolates in the studied hospitals.
-Methicillin resistant Staphylococcus aureus, Cefoxitin, SCCmec, Multiplex PCR, femB gene, mecA gene
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ISSN 1608-5671 (Print)  

ISSN 2008-160X (Online)  
 
     
     
 

ضريب تأثير (IF) مجله پزشکی دانشگاه علوم پزشکی تبریز  در  پايگاه  استنادي علوم جهان اسلام (ISC)  

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