پنجشنبه 2 آذر 1396
به سایت مجله پزشکی دانشگاه علوم پزشکی و خدمات بهداشتی و درمانی تبریز خوش آمدید
   نوامبر 23, 2017
   
   
     
  تعداد اعضاء : 2866 نفر
  بازدیدهای امروز :214 بار
  تعداد کل بازدیدها : 968128 بار
   
Volume: 38
Year: 2016
Month: 11
Subject Others
Title Comparison Three Methods, PCR Method, Cultyre and Rapid Urease Test to Detect Helicobacter pylori in the Gastric Biopsy Tissue Samples
Authors Mohammad reza Nafisi; Mohammad Alipour Shadbad; Gorban ali Rahimian; Ali Karimi;
Abstract Background & Objectives: Helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations. The aim of this descriptive study was to evaluate diagnostic methods, such as rapid urease test, culture and polymerase chain reaction (PCR). Materials and Methods: For identification of Helicobacter pylori 100 patients (46 women and 54 men) who were suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord were participated in the study. Gastric biopsy samples were collected from each patient, then polymerase chain reaction, culture and rapid urease test were performed. DNA extraction was followed by PCR was used for diagnosis of ureC gene. Results: Biopsy samples from100 patients were evaluated. H. pylori was positively identified by PCR in 78/100 (78%) of the patients, while positive samples were found in 52 (52%) and 48 (48%) of the patients by RUT and culture methods, respectively. In results of the culture, there was an agreement of 100% with PCR. Conclusion: Three different methods for the detection of Helicobacter pylori were evaluated. Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid urea as test, therefore this method could be used to detect H.pylori.
-Helicobacter pylori, Rapid Urease Test, Culture, Ure C(gene), PCR
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ISSN 1608-5671 (Print)  

ISSN 2008-160X (Online)  
 
     
     
 

ضريب تأثير (IF) مجله پزشکی دانشگاه علوم پزشکی تبریز  در  پايگاه  استنادي علوم جهان اسلام (ISC)  

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