سه شنبه 30 آبان 1396
به سایت مجله پزشکی دانشگاه علوم پزشکی و خدمات بهداشتی و درمانی تبریز خوش آمدید
   نوامبر 21, 2017
   
   
     
  تعداد اعضاء : 2866 نفر
  بازدیدهای امروز :84 بار
  تعداد کل بازدیدها : 966957 بار
   
Volume: 39
Year: 2017
Month: 7
Subject Others
Title Prevalence of CTX-3 family gene among clinical isolates of Escherichia coli and Klebsiella pneumoniae from patients hospitalized in Sina Hospital, Tabriz
Authors Ali Pormohammad; Alka Hasani; Mohammad Aghazadeh; Mohammad Ahangarzadeh Rezaee; Akbar Hasani; Mohammad Reza Nahaei; Froogh Shams; Akbar Mohammadzadeh;
Abstract Background: Emerging resistance to beta-lactam antibiotics among gram negative bacteria limits their usage. This study was done to determine the frequency of ESBLs producers and presence of CTX-M3 family gene (including CTX-M 3, 15, 22 subfamily) in Escherichia coli and Klebsiella pneumoniae isolated from different clinical specimens in Sina Hospital, Tabriz Methods: 71 isolates of E. coli and 63 K. pneumoniae were isolated from different clinical specimens sent to Division of Microbiology, Sina Hospital, Tabriz, Iran. Bacteria were identified by conventional phenotypic methods. ESBL production in E. coli and K. pneumoniae was first detected with combined disc method using Mueller-Hinton agar and later presence of CTX-M3 family gene was detected by PCR technique. Results: In this study, 41 (57.74%) E.coli and 45 (71.42%) K. pneumoniae isolates were observed as ESBL producers. Among them, 30 (73.17%) E. coli and 26 (57.77%) K.pneumoniae were found carrying CTX-M3 gene. Among various antibiotics used for ESBL detection, highest resistance towards cefpodoxime (92%) was observed in E.coli, while in K.pneumoniae 90% isolates show resistance towards cefpodoxime and azterornam. Conclusion: Our study revealed that there is a high frequency of ESBLs producing isolates of E. coli and K. pneumoniae in our hospital set up. The problem elucidates the importance of designing more controlled surveillance of antibiotic resistance and need for large-scale epidemiologic studies to identify outcomes of the ESBL-production in gram negative bacilli.
-E.coli, Klebsiella pneumoniae, Extended Spectrum beta- lactamase, CTX-3
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ISSN 1608-5671 (Print)  

ISSN 2008-160X (Online)  
 
     
     
 

ضريب تأثير (IF) مجله پزشکی دانشگاه علوم پزشکی تبریز  در  پايگاه  استنادي علوم جهان اسلام (ISC)  

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