دوشنبه 2 مهر 1397
به سایت مجله پزشکی دانشگاه علوم پزشکی و خدمات بهداشتی و درمانی تبریز خوش آمدید
   سپتامبر 24, 2018
   
   
     
  تعداد اعضاء : 3116 نفر
  بازدیدهای امروز :222 بار
  تعداد کل بازدیدها : 194407 بار
   
Volume: 40
Year: 2018
Month: 2
Subject Others
Title Optimization of refolding of human vascular endothelial growth factor-A121
Authors Fatemeh Kazemi-Lomedasht; Mahdi Behdani; Kamran Pooshang Bagheri; Delavar Shahbazzadeh; Mahdi Habibi-Anbouhi;
Abstract Background: Vascular endothelial growth factor (VEGF) plays an important role in development of new blood vessel and angiogenesis. Human VEGF121 is smallest member of VEGF family. Production of active and correct form of VEGF is the most challenging issue. Methods: Here we described a method for optimization of refolding of VEGF121 which was expressed in bacterial host. Enzyme linked immunosorbent assay and proliferation assay of human endothelial cells was performed to monitor refolding and functional assay of VEGF121. Results: Using described method; VEGF was in correct fold and detected by antibody in ELISA. Furthermore, VEGF stimulated proliferation of human endothelial cells in dose-dependent manner. Conclusion: Refolded VEGF has potential for stem cell differentiation.
Keywords: Vascular Endothelial Growth Factor, Angiogenesis, Refolding
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ISSN 1608-5671 (Print)  

ISSN 2008-160X (Online)  
 
     
     
 

ضريب تأثير (IF) مجله پزشکی دانشگاه علوم پزشکی تبریز  در  پايگاه  استنادي علوم جهان اسلام (ISC)  

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