يکشنبه 4 اسفند 1398
به سایت مجله پزشکی دانشگاه علوم پزشکی و خدمات بهداشتی و درمانی تبریز خوش آمدید
   فيفرييه 23, 2020
   
   
     
  تعداد اعضاء : 3802 نفر
  بازدیدهای امروز :328 بار
  تعداد کل بازدیدها : 637323 بار
   
Volume: 41
Year: 2019
Month: 12
Subject Others
Title The Study of NF-KB and MAPK Genes Expression in HT29 Colon Cancer Cell line Co-Cultured with Streptococcusthermophilus
Authors Mahdiyeh Behrouz Sarand; Changiz Ahmadizadeh;
Abstract Background: Probiotics are normal flora bacteria. So far, variousmechanisms have been identified for the effects of probiotics such as stimulating the immune system, modulating the composition of the normal flora of the digestive, urinaryand genital tract, and preventing the activity of fecal carcinogenicenzymes. Due to the high concentration ofnormal flora in the intestine and the nature of the most sporadicof colorectal cancers, this cancer is among the main candidates fortreatment with probiotics. In this study, the expression of NF-KB and MAPK genes were investigated HT29 colon cancer cells which co-cultered with Streptococcusthermophilus. Methods: Supernatant and bacterial extract wereprepared from bacterial culture, and the cells were treated with these agents. Following, the effects ofcytotoxicity of the bacterial cell extract on the HT29 cell line during24hours were investigated using MTT method. Also, the expression ofNF-KB and MAPK genes in HT29 cell line was investigated using Real TimePCR Data were analyzed by one-way ANOVA. Results: The results of MTT test showed that concentration of bacteria with OD= 0.05 had thehighest killing power in 4 hours. Streptococcus thermophilus increases the expression of MAPK genes and significantly reduces theexpression of NF-KB genes and causes apoptosis in cancer cells. Conclusion: The results of this study showed that Streptococcusthermophiluscan be used to create a novel therapeutic treatment with highimpact, low side effects, bioavailability and cost reduction, or as asuitable side therapy.
Keyword: NF-KB, MAPK, Co-culturing, Streptococcus thermophiles, Colon Cancer HT29
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ISSN 1608-5671 (Print)  

ISSN 2008-160X (Online)  
 
     
     
 

ضريب تأثير (IF) مجله پزشکی دانشگاه علوم پزشکی تبریز  در  پايگاه  استنادي علوم جهان اسلام (ISC)  

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